A Low-Cost Efficient Multiplex P CR for Prenatal Sex Determination in Bovine Fetus U sing Free Fetal DNA in Maternal Plasma
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a low-cost efficient multiplex p cr for prenatal sex determination in bovine fetus u sing free fetal dna in maternal plasma
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full textA Low-Cost Efficient Multiplex PCR for Prenatal Sex Determination in Bovine Fetus Using Free Fetal DNA in Maternal Plasma
BACKGROUND In order to establish a reliable non-invasive method for sex determination in a bovine fetus in a routine setting, the possibility of identifying specific sequence in the fetal X and Y-chromosomes has been evaluated in maternal plasma using conventional multiplex polymerase chain reaction (PCR) analysis. The aim of this study was to provide a rapid and reliable method for sexing bovi...
full textPrenatal determination of farm animal fetal sex using free fetal DNA in maternal plasma
In many farm animal species, the preference of a specific sex is obvious mainly due to commercial implications; so for comprehensive and efficient results in the livestock management plans specially breedingand selection programs, it is necessary to include acost-effective and reliable strategy for early determination of fetal sex. Several invasive and non-invasive approaches have been establis...
full textEvaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma
OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...
full texthighly sensitive conventional pcr testing of free fetal dna in maternal serum for non-invasive fetal sex determination
background: free fetal dna (ffd) in maternal plasma/serum has increasingly become the source of fetal material for diagnostic purposes in recent years. this source of fetal material can be used for sex determination. rh typing paternally inherited sequences and compound heterozygosity. reports on the lack of consistent pcr amplification of y-chromosome sequences of ffd in maternal plasma/serum ...
full textevaluation of two dna extraction methods from maternal plasma for using in non-invasive bovine fetus gender determination
background: fetal dna in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. non-invasive prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing. objective: the purpose of this study was to evaluate two dna extraction methods from mother plasma and its routine clinical applica...
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Journal title
volume 6 issue 1
pages 45- 50
publication date 2012-06-01
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